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MOLECULAR
PROTOCOLS:
Antisense probe transcription
Autorad
protocol 9 Mar 04
BAC
miniprep protocol
BAC
Purification
BES
Blunt
Ending DNA fragments
Buffer
Compatibility Cheat Sheet
Buffers
Cheat Sheet
DNA
Molecular Weight Standards
DNA
Gel Purification
GAD65
KO PCR protocol
Guidelines
for Use and Storage of PCR Primers
Highly
Efficient Ligation for Large Inserts
How
to find BAC clones
In
situ protocol (Lisa's changes)Fluorescent Insitu Hybridization (FISH)
Flourescent Insitu Hybridization (FISH)
ISH (Free Floating)
ISH reagents
Modified
MinElute protocol for DNA frag purification
Plasmid
MaxiPrep
RadiationMAP
Reconditioning
electroporation cuvettes
Southern
Blotting
Southern Blotting (Zoe's new protocol)
Transgenic
tail prep & PCR
Ear Punch DNA prep
Vector
NTI shortcuts / guide
IMMUNO:
FOS
floating section protocol #1
FOS floating protocol -Murphy lab
anti-GFP
ICC protocol
general
floating section ICC
Western
Blotting (Lisa)
Western
Blotting
HISTOLOGY:
Cryostat
Rules
Emulsion
Coating Autorad and in situ slides
ICC
Protocol Floating Sections
Staining
for LacZ with Xgal
LENTIVIRUS PROTOCOLS:
Lentivirus
Modified
MinElute protocol for purifying DNA fragments
Viral
Vector production
CELL CULTURE:
Counting
dissociated cells
Lipofectamine
transfection
PC12
differentiation assay
BEHAVIOR (Mice):
Analysis
of SR-generated Freezing and FPS data
Basic
Fear Conditioning Protocol
Maternal
Separation
Object
Discrimination Protocol
Object
Recognition (Tang Study protocol)
Object
Recognition (pictures)
Open
Field and Elevated Plus Maze
Open
Field software
SURGERY / DRUGS:
Anesthetic
Drug Recipes
OTHER (Human):
SCID
scoresheet
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