The Emory University Mass Spectrometry Center

 The Emory University Mass Spectrometry Center is a laboratory that provides mass spectrometry service to the Emory University community. It is located in Room 157 of the Stanford S. Atwood Chemistry Center. Although it is located in the Chemistry Center, the Emory University Mass Spectrometry Center is for the entire university community.

The Center has two instruments available. The first instrument is a JEOL JMS-SX102/SX102A/E mass spectrometer. The JEOL is a five-sector tandem mass spectrometer. The JEOL provides many unique capabilities such as multiple dimensional mass spectrometry, which may be used for structure elucidation. The second instrument is a Voyager -STR MALDI Time-of-flight mass spectrometer which provides unique capabilities for biomolecules. This instrument is used by the students themselves.

The routine analyses that are performed in the Center are electron ionization (EI), electrospray ionization (ESI), fast atom bombardment (FAB), and liquid secondary ion mass spectrometry (LSIMS). High-resolution analysis is available using EI and FAB. EI is commonly used for small-mass volatile compounds (<500 daltons). FAB and LSIMS are commonly used for higher mass compounds up to approximately 3000 daltons. ESI is also commonly used for high mass compounds (up to 50,000 daltons). In particular, ESI can be used to study proteins and other very large molecules. Maldi is used for large molecules (molecular weights > 100,000 daltons can be measured) plus high sensitivity work on intermediate size molecules (> 500 daltons)

Structural information can be obtained from multiple MS experiments. These experiments analyze fragments produced by a selected ion. The unique geometry of the JEOL JMS-SX102/SX102A/E allows for the analysis of 5th generation products. The Voyager can also obtain structural data by an experimental method called post-source decay.

To get a sample analyzed, the samples are brought to the Center and a submission sheet is filled out. A copy of the submission sheet may be obtained from the center personnel. The analysis is performed by the Center personnel. Typically, the samples are analyzed in less than 1 week (Average is 4 days). The Center director is available to answer questions about the type of experiments that can be done and the best method of analysis.

To get trained on the MALDI, contact the director of the mass spectrometry

More information about mass spectrometry can be found by searching base-peak mass spectrometry site..

Any further questions can be directed to the Center Director, Dr. Strobel (fstrobe@emory.edu).

SERVICES

Routine Services and internal prices

  • Low resolution electron ionization-$12.00
  • High resolution electron ionization -$24.00
  • Low resolution fast atom bombardment-$12.00
  • High resolution fast atom bombardment -$24.00
  • Electrospray ionization-$24.00
  • MALDI -($7.50/hr for user run samples, and $30.00 per sample)

Other Services (internal price $24.00/hr)

  • Nanoelectrospray and MS/MS
  • Chemical ionization
  • MS/MS
  • Multiple MS experiments
  • Mass-analyzed ion kinetic energy spectrometry
  • Other (contact the Director for further information(fstrobe@emory.edu))

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SAMPLE SUBMISSION

The sample and a sample submission form are submitted to room 157 of the Stanford S. Atwood Chemistry Center.

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INSTRUMENTS OF THE EMORY UNIVERSITY MASS SPECTROMETRY CENTER

Personnel of The Emory University Mass Spectrometry Center

 

Director

 

Service Instructors

 

    • Uliana Danilenko

 

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Any further questions can be directed to the Center Director, Dr. Strobel (fstrobe@emory.edu).